Spinach extract contains a 20 and a 25 kDa protein, as well as 14-18 kDa proteins (minor bands on blot). One or more proteins appear to be heat-stable (1). A cross-reactive 30 kDa protein has also been detected - The following allergen has been characterised: Spi o 2, a profilin (3).
No specific preparation required but clinical history format is mandatory.
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