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Factor IX activity is determined utilizing an aPTT-based one-stage clotting time assay. Factor IX-depleted plasma is used as the substrate, and the clotting time with the patient plasma is compared to the clotting time of normal pooled plasma.patient with congenital deficiency of factor IX are said to be suffering from bleeding disorder: Hemophilia B
Overnight fasting is required. Before to sampling, it is advised that the patient stop using oral anticoagulants for seven days and heparin for one day, respectively. Discontinuation should only be done with the treating physician permission.