Overnight fasting is required. Before to sampling, it is advised that the patient stop using oral anticoagulants for seven days and heparin for one day, respectively. Discontinuation should only be done with the treating physician permission. Kindly keep note: For Coagulation assay, individual separated citrate plasma tube required, minimum 1ml each if more than one factor assay is required in view of batch assay of some of the parameters.