Preperations Required 24 hour Urine should be collected in mug and transferred to relevant Urine container with or without preservative. 20 ml of aliqout to be send to lab in refrigerated condition along with clinical history and mention Urine volume of 24 Hr collected sample for calculation purpose. Time of collection, start time to end time and exclude frst urine sample and include last voided urine sample. Many analytes like VMA, 5 HIAA, adrenaline may require drug avoidance as well as bananas, tea , coffee , cold drink avoidance for accurate results and such historyto be conveyed to lab.