Preperations Required Collect samples in a Royal blue K2EDTA (whole blood) trace element 6 ml tube. Never transport specimens in glass collection tubes.Avoid collecting the specimen in an area where environmental contamination is likely to occur. Use only stainless steel needles and non-powdered gloves when handling and collecting. Wash the collection site with soap and water, followed by an alcohol swab. Do not use disinfectants containing iodine. When multiple blood specimens need to be collected from one patient, the trace elements sample should be the first tube collected