Preperations Required 24 H urine: 24 hour Urine should be collected in mug and transferred to relevant Urine container without preservative. 20 ml of aliqout to be send to lab in refrigerated condition along with clinical history and mention Urine volume of 24 Hr collected sample for calculation purpose. Time of collection, start time to end time and exclude first urine sample and include last voided urine sample. Mention 24 Hrs.Urine Volume.10 ml of 24 Hours Urine In Dark Container. No Preservative