THROMBIN TIME (O.S)

Requirements

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    Sample Required SODIUM CITRATE PLASMA

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    Preperations Required Clinical history is mandatory Overnight fasting is preferred. A duly filled Coagulation Requisition Form (Form 15) is MANDATORY. It is recommended that the patient stops Heparin 1 day before sampling and Oral Anticoagulants 7 days before, as these medications can affect the test results. Discontinuation should only be done with prior consent from the treating PHYSICIAN.

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    Gender Male & Female

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    Age Group 0 - 99 Years

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    Collection AtHome & Lab

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    1 Observations included

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THROMBIN TIME (O.S)

Observations Included

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Overview

Thrombin catalyzes the transformation of fibrinogen to fibrin which is followed by polymerization of fibrin to form a clot. The thrombin time (TT) test measures the time of clot formation when thrombin is added to citrated plasma .Prolongation of the thrombin time (TT) is consistent with the presence of heparin-like anticoagulants, hypofibrinogenemia, dysfibrinogenemia, fibrin degradation products, and antibody inhibitors of thrombin. An immeasurably prolonged TT is usually the result of heparin in the specimen or, rarely, the presence of thrombin antibodies or afibrinogenemia. Draw 6 mL of whole blood in 2 blue-top (Sodium Citrate) tube and gently mix by inverting. Transport the sample to the lab within 4 hours. If this isn’t possible, prepare PPP within 1 hour: First, centrifuge the sample at 3600 rpm for 15 minutes, then transfer the supernatant to a clean plastic tube. Centrifuge this supernatant again at 3600 rpm for another 15 minutes, and transfer the final supernatant (PPP) into a LABELED, clean plastic screw-capped vial. FREEZE IMMEDIATELY and ship FROZEN. DO NOT THAW.

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