This test is a qualitative in vitro test for the single or multiple detection of Influenza A virus (Flu A), Influenza B virus (Flu B), Human Influenza A virus subtype H1 (Flu A-H1), Human Influenza A virus subtype H3 (Flu A-H3), and Human Influenza A virus subtype H1pdm09 (Flu A-H1pdm09) from clinical samples.Symptoms for having influenza is nasal discharge, nasal congestion, sore throat, cough, fever, nasopharyngitis, chill and muscle pain, which are severe than regular cold. Infection with H1N1 and H3N2 subtypes of influenza A virus poses serious threat for respiratory collapse.