It is used for Detection of previous exposure or immunity to hepatitis A infection. The virus is transmitted primarily by the fecal-oral route and is spread by close person-to-person contact as well as by food- and water-borne epidemics. A negative result indicates the absence of HAV-specific IgG antibody, implying no past exposure or immunity to HAV infection. A positive result indicates the presence of HAV-specific IgG antibody from either vaccination or past exposure to hepatitis A virus.