Prothrombin time is used to identify a deficiency of one or more of the clotting factors of the extrinsic coagulation system (I, II, V, VII, X) due to hereditary deficiency or acquired conditions such as liver disease, vitamin K deficiency, or a specific factor inhibitor. Monitoring patients on oral anticoagulant therapy to maintain a patient in a safe therapeutic range.The prothrombin time is also reported as the INR (International Normalized Ratio), based on the ISI (International Sensitivity Index) Draw 6 mL of whole blood in 2 blue-top (Sodium Citrate) tube and gently mix by inverting. Transport the sample to the lab within 4 hours. If this isn’t possible, prepare PPP within 1 hour: First, centrifuge the sample at 3600 rpm for 15 minutes, then transfer the supernatant to a clean plastic tube. Centrifuge this supernatant again at 3600 rpm for another 15 minutes, and transfer the final supernatant (PPP) into a LABELED, clean plastic screw-capped vial. FREEZE IMMEDIATELY and ship FROZEN. DO NOT THAW.