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The Vitamin D (25 Hydroxy) Test is a blood test used to measure the level of vitamin D in your body. It helps detect vitamin D deficiency or excess and is considered the most accurate test for checking vitamin D status.
Doctors may recommend this test if you have symptoms such as fatigue, bone pain, muscle weakness, frequent illness, or low immunity. It is also commonly advised for people with limited sun exposure, osteoporosis, or suspected vitamin D deficiency.
Usually, fasting is not required for a Vitamin D (25 OH) blood test. However, your doctor or laboratory may provide special instructions if the test is combined with other blood investigations.
Low vitamin D levels may cause tiredness, bone or back pain, muscle weakness, hair fall, mood changes, and frequent infections. In severe cases, it can affect bone strength and overall immunity.
Vitamin D levels are generally considered normal between 20–50 ng/mL, though the ideal range may vary slightly depending on the laboratory and your health condition. Your doctor will interpret the results based on your overall health.
This test requires a simple blood sample collected from a vein in your arm. The procedure is quick, safe, and usually completed within a few minutes.
People with low sunlight exposure, older adults, pregnant women, individuals with bone disorders, obesity, digestive disorders, or those taking vitamin D supplements may need regular monitoring through this test.
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| Vitamin D Level | Range (ng/mL) |
| Deficient | Less than 20 |
| Insufficient | 20 – 30 |
| Sufficient | 30 – 100 |
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